Molecular imprinting covalent approach

Molecularly imprinted polymers (MIPs) can be prepared according to a number of approaches that are different in the way the template is linked to the functional monomer and subsequently to the polymeric binding sites (Fig. 6-1). Thus, the template can be linked and subsequently recognized by virtually any combination of cleavable covalent bonds, metal ion co-ordination or noncovalent bonds. The first example of molecular imprinting of organic network polymers introduced by Wulff was based on a covalent attachment strategy i.e. covalent monomer-template, covalent polymer-template [12]. 

Fig. 1. Concept of molecular imprinting - the non-covalent approach. 1. Self-assembly of template with functional monomers. 2. Polymerization in the presence of a cross-linker. 3. Extraction of the template from the imprinted polymer network. 4. Selective recognition of the template molecule...

Other Immobilization Techniques Along with noncovalent and covalent immobilization methods, other techniques have been developed for the preperation of affinity supports. Such methods include entrapment, molecular imprinting, and the use of the ligands as both the support and stationary phase. Although these methods are not as common as the approaches already examined, they have important advantages in some applications [8]. 

Sellergren B (2001) The non-covalent approach to molecular imprinting. In Sellergren B (ed) Molecularly imprinted polymers. Man made mimics of antibodies and their applications in analytical chemistry. Elsevier, Amsterdam... 

A novel approach to guest inclusion by dendrimers is the use of molecular imprinting . We will look at molecular imprinted polymers in more detail in Section 14.3. Generally the imprinting technique involves assembly of a covalent polymer - in this case dendrimer - about the target guest which acts... 

Fig. 1.8. The covalent approach to molecular imprinting in organic polymers, as introduced by the group of Wulff, exemplified by the o-glyceraldehyde MIP reported in 1972 [1],...

The non-covalent approach to molecular imprinting Choice of the liinctiona] monomer... 

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