Molecular Combing as a General Technique

In this chapter, we try to present a mechanistic view of molecular combing so that this process can be adapted to new applications. The theoretical formulation for deposition and alignment, which we develop further here, originally described the deposition and alignment of DNA-SWCNTs. Due to the similarities between dsDNA and DNA-SWCNTs, the theory should be applicable to both cases. 

Molecular combing occurs in two steps, the deposition of the object on the surface and its alignment by a passing meniscus (Fig. 16.1a,b). We discuss first the deposition and then the alignment, addressing the techniques that have been developed to control both processes. The theoretical framework developed for the system will be presented. 

Bensimon etaL, Bacteriophage X DNA combing studies SAM of vinyl-tBrminated alkenes on Over 70% of DNA molecules extended by combing density of molecules  

DNA (lO bp) ass increases with DNA concentration and at low pH (where surface charge 

Bensimon etal, LongdsDNA SAM of vinyl-terminated alkenes. is reduced). DNA attached at both ends results in loops combed DNA can be 

---