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Molecular beacons excimer-monomer switching

Fujimoto K, Shimizu H, Inouye M (2004) Unambiguous detection of target DNAs by excimer—monomer switching molecular beacons. J Org Chem 69 3271-3275... [Pg.60]

Figure 2.8a. Schematic representation of excimer-monomer switching molecular beacons (EMS MBs). EMS-MBs were dually labeled with pyrene at both 3 and S ends of single-stranded oligonucleotides with a stem-and-loop structure. In tlie absence of target DNAs. the slem-close-shaped EMS MBs predominantly emit the exclmer fluorescence (yellow-green). Upon hybridization with target DNAs, the EMS-MBs undergo the ilynamic conformational change to emit the monoincr fluorescence (pale Uue). Figure 2.8a. Schematic representation of excimer-monomer switching molecular beacons (EMS MBs). EMS-MBs were dually labeled with pyrene at both 3 and S ends of single-stranded oligonucleotides with a stem-and-loop structure. In tlie absence of target DNAs. the slem-close-shaped EMS MBs predominantly emit the exclmer fluorescence (yellow-green). Upon hybridization with target DNAs, the EMS-MBs undergo the ilynamic conformational change to emit the monoincr fluorescence (pale Uue).
Froschle, M., Ulmer, W. and Jany, K. D. 1984, Tyrosine modification of glucose dehydrogenase from bacillus megaterium. Effect of tetranitromethane on the enzyme in the tetrameric and monomeric state. European Journal of Biochemistry 142, 533-540. Fujimoto, K., Shimizu, H. and Inouye, M. 2004, Unambiguous Detection of Target DNAs by Excimer-Monomer Switching Molecular Beacons. Journal of Organic Chemistry, 69, 3271 -3275. [Pg.393]

See also in sourсe #XX -- [ Pg.273 ]



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