Molecular beacons probes

Tyagi S, Kramer FR (1996) Molecular beacons probes that fluoresce upon hybridization. Nat Biotechnol 14 303-308... 

Tyagi, S. Kramer, F. R., Molecular beacons Probes that fluoresce upon hybridization, Nature Biotechnol. 1996, 14, 303 308... 

APTS (for sugar isomers) [560], RNA molecular beacon probes [337], and YOPRO-1 (for dsDNA) [613]. In one report, a ring of 24 blue LEDs (470 nm) has been used for flood illumination [321]. 

In another report, real-time NASBA was used to amplify RNA in a Si-Pyrex chip containing 50-nL reaction chambers. This is an isothermal (41°C) technique. A fluorescent molecular beacon probe was used to detect the RNA derived from the human papillomavirus (HPV16) [337]. 

Thomas A, Smith HR, Willshaw GA, Rowe B (1991) Non-radioactively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VTl, VT2 and VT2 variant. Mol Cell Probes 5 129-135 Thompson JS, Hodge DS, Borczyk AA (1990) Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype 0157. J Chn Microbiol 28 2165-2168 Tyagi S, Kramer FR (1996) Molecular beacons Probes that fluoresce upon hybridization. Nat Biotechnol 14 303-308... 

Figure 16.6. Schematic representation of molecular beacon probes reaction with target DNA sequence. Copyright 2005 and adapted with the kind permission of Invitrogen Corporation.

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