Mitochondrial assays transmembrane potential

At the end of treatment, HL-60 cells were stained with 40 nM 3,3 -dihexyloxacarbocyanine (DiOC6 (3), Molecular Probes, Eugene, OR) for 15 min at 37 C. Mitochondrial transmembrane potential was measured by flow cytometry. At least 10,000 cells were collected in each sample. Generation of ROS was monitored with 20 pM 2, 7 -dichlorofluorescein diacetate (DCFH-DA) (Molecular Probes, Eugene, OR) staining for 1 h at 37 C and assayed by flow cytometry. 

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