Millisecond HX Kinetics

there is much to gain from the development of an analytical framework that is specifically suited for millisecond FIX. At present, however, owing mainly to the limited use of millisecond exchange and the requirement for slightly shorter labeling pulses than are used even in most millisecond HX experiments, none of the potential benefits described previously have yet been exploited. 

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Figure 5.10 Millisecond HX kinetics revealing loop dynamics in native cytochrome c. (Left) The observed rate for each loop Is compared to the chemical exchange rate in order to generate a segment-averaged protection factor. (Right) Loop flexibility of cytochrome c, mapped on to pdb lAKK [53], with darkest to lightest gray indicating least to most flexibility. Reproduced with permission from Ref [21]. 2012, American Chemical Society. (See insert for color representation of the figure.)...

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