Millilab workstation

Table 26.1 Average Recovery of 10 ng/pl Standard Containing Organic FDR by SPE on the Millilab Workstation...

The Chromosorb 104-Amberlite XAD-4 SPE column prepared in the laboratory allows full automation of the extraction process on the Millilab workstation. Subsequent experiments were performed using these SPE columns. 

The efficiency of extraction of organic FDR from the Deldrin unit and the subsequent SPE cleanup and concentration using the Millilab workstation were assessed. The results are listed in Table 26.2. 

It was found that the recovery of organic FDR was reduced when the Deldrin filter unit was used (57% to 78% recovery compared to 95% recovery from the SPE columns). This may be explained by the presence of garment fibers and debris recovered with the FDR. The more material present within the Deldrin unit, the more difficult it is to extract the FDR with a given volume of acetonitrile (total extract 1.4 ml). Using a greater volume of acetonitrile poses problems with the subsequent 1 9 dilution of extracts from SPE. The Millilab workstation is limited to using 160 x 10 mm tubes for dilution (a total workable volume of 14 ml). 

Table 26.2 Recovery of Organic FDR from Deldrin/SPE Units Using the Millilab Workstation...

The Millilab 1A workstation is a personal computer-controlled automated robotic system which performs sample extraction from filters and SPE devices according to user-defined programs. This was used to compare the efficiency of different SPE materials, as manual error is substantially reduced. The results are listed in Table 26.1. 

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