Milk powder blocking agent

On blots blocked with milk powder (5%), the ECL reaction apparently runs only weakly. Blots blocked with gelatin, on the other hand, exhibit a good ECL reaction, but also a higher background. The best blocking agents for ECL blots are said to be serums. Important acid inhibits peroxidases. 

Skimmed milk powder can replace blocking agent in this buffer. We have used Marvel at a final concentration of 1 %. Marvel milk powder, surprisingly, should be stored frozen. 

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