Microsomes avocado

The incorporation of [ C]glycerol and [ CJglycerol phosphate into lipids was studied by Barron and Stumpf (1962). The active fraction obtained from avocado Persea americana L.) fruit mesocarp was purified by density gradient centrifugation, and most of the activity was found in the microsomal fraction. The first product was PA [but analysis at early time points detected lysophosphatidic acid (LPA)], followed by monoacylglycerol, diacylglycerol, and triacylglycerol in that order. 

PAP has been purified from yeast [2], and animal tissue [3,4,5]. We have purified microsomal PAP to homogeneity from avocado (Persea Americana) and report here enzymological properties for solubilised PAP. 

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