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Multicontrast microscopy

VIII. Multicontrast Nonlinear Microscopy of H E Stained Histological... [Pg.71]

This chapter introduces principles of three nonlinear contrast mechanisms— MPF, SHG, and THG—for microscopic imaging. The instrumentation of nonlinear multicontrast microscopy is described, and the methods of multicontrast image analysis are presented. The later parts of the chapter focus on several examples of multicontrast nonlinear microscopy applications in biological imaging. [Pg.73]

SCIA can be applied for correlation of three images obtained with multicontrast nonlinear microscopy. The two-image SCIA can be applied to correlate separately each two of the three images. However, direct correlation of all three images with SCIA algorithm renders quicker and more transparent results. To illustrate three-channel SCIA, an example is shown in Figure 4.2 where three channels (A, B, and C)... [Pg.85]

VIII. MULTICONTRAST NONLINEAR MICROSCOPY OF H E STAINED HISTOLOGICAL SECTIONS... [Pg.95]

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Multicontrast nonlinear microscopy

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