Microscope, binocular dissecting

Slice dissection can be carried out either in a Pyrex Petri dish or on a bed of agar. In both cases dissection is performed in aCSF cooled to on ice A binocular dissecting microscope is helpful for accurate dissection. 

Binocular dissecting-type microscope, capable of 40x magnification. 

Stereoscopic binocular dissecting microscope—transmitted light is preferable. 

Microscopes For electroporation, a binocular dissection microscope and fiberoptic light source. For monitoring GFP fluorescence, a fluorescence dissection microscope. 

Use a binocular dissecting microscope to analyze and photograph the finished embryos (see Note 20). 

While observing the CNS under a binocular dissecting microscope, add 1 il of to the solution in the watch glass and observe the resulting reaction. 

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