Microperoxidase conjugation

The bifunctional ester bissuccinic acid A-hydroxysuccinimic ester (BSNHS) is used for conjugation of microperoxidase to antibodies. It gives high antibody activity because 8-microperoxidase has only one reactive amino group. 

Chemiluminescence-labelling has been applied to the immunoassay of oestriol 16a-glucuronide by synthesis of the A-aminobutyl-A-ethylisoluminol derivative (30), which emits light when oxidized with H202-microperoxidase. Binding of the conjugate (30) to an antibody for the steroid enhances light emission. Competitive... 

Conjugation of microperoxidase to antibodies by bis-succinic acid 7V-hydroxysuccinim-ic ester (Ryan et al.. 1976)... 

Some effort has been devoted to obtain even smaller probes, i.e., smaller enzymes conjugated to Fab. An early candidate was cytochrome c (Singer, 1974) which has only 1/3 of the molecular weight of POase, though its diameter is only 30% smaller, but it has the disadvantage of low enzymatic activity. Digestion of cytochrome c (Section 10.3.1) yields the active site (microperoxidase, MPOase)... 

Avidin was reacted with compound 9 in the presence of NHS and EDAC to yield a conjugate with 16 Fc imits bound per protein molecule. This Fc-tethered conjugate was immobilized in a covalent manner on a SAM of DTSP on a gold electrode. This resulting redox layer was in turn used as a platform to immobilize, by a bioaffinity process, several biotinylated enzymes, including microperoxidase... 

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