Microinjection injection

Much evidence supports this scheme. For example, neuronal depolarisation increases the amount of free synapsin in the cytosol and microinjection of CAM kinase II into the terminals of the squid giant axon or brain synaptosomes increases depolarisation-evoked transmitter release. By contrast, injection of dephosphorylated synapsin I into either the squid giant axon or goldfish Mauthner neurons inhibits transmitter release. 

RNA transcript combinations are prepared for injection by adding a small volume (0.5-1.0 pL) of the cRNA(s) encoding each required subunit to a 1.5 mL microfuge tube. Individual subunit cRNAs are combined and pulse spun for a few seconds and kept on ice until ready for injection. Preparation of a suitable pipette for microinjection of transcripts is imperative to ensure proper injection with minimum damage to the oocyte membrane. 

Microinjection No limit myocytes Mitosis/resting at injection site Stable <0.1-1% Integration possible infiltration 30% survival 200-400 injections/hr In vitro... 

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