Microgels solution mobility

In contrast to HPLC, where polar effects dominate molecule separation, gel permeation chromatography (GPC) separates molecules according to their molecular size. Types of stationary phase and eluents used for GPC are HIBAR columns (Merck) [33] and Microgel (Chrompack) columns [215] of different pore size with tetrahydrofurane as eluent [33,214,215] Sephadex LH 20 gel and a sodium acetate solution [216] orN,N-dimethylformamide [217] as mobile phase Styragel columns and dimethylformamide eluent containing 2% acetic acid [218] and micro-packed, fiised-silica columns with tetiahydrofiirane as eluent [219]. Detection is by ultraviolet absorbance at the same wavelength discussed in HPLC analysis. 

Where 11 and II are, respectively, the osmotic pressure of the mobile ions inside the microgels and bulk solution. II ei is the elastic pressure of the polymeric network [73]. 

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