Microfluidic design

Figure 5.1 Top a schematic of how to translate the chemical reaction steps of the synthesis of 2-deoxy-2-fluoro-D-glucose into a microfluidic design with microchannels and valves for consecutive fluid transport und manipulation...

FIGURE 16.25 Microfluidic design for sample injection from a twin-T channel geometry. (Reprinted with permission from Effenhauser, C.S., et al., Anal. Chem., 66, 2949, 1994. Copyright 1994 American Chemical Society.)... 

Although the theoretical studies on this subject lag the experimental ones, some theoretical studies have been accomplished recently to discover the nature of the membrane permeabilization and cell transfection. It helps the experimentalist to optimize the design and performance of their proposed microfluidic design for electroporation. 

An application of AC electrokinetic phenomena is the preconcentration of a target molecule within a small volume. Crooks and coworkers (88) have achieved enrichment factors for DNA exceeding 100 using channels that are 5 mm in length, require only a single homogeneous analyte phase, and avoid complex microfluidic designs. The approach... 

Pumera, M. (2007) Contactless conductivity detection for microfluidics designs and applications. Talanta, 74 (3), 358-364. 

Figure 9.30 Representation of the microfluidic design to create aqueous droplets containing cells in oil flow. White light and FT-IR images obtained in transmission with and without the pseudosphere employed to correct chromatic aberration. Note that the FT-IR transmission images are plotted using the...

Contrary to our intuition in the macroscopic world, fluids do not mix, bugs can walk on water, and our approach for swimming does not work. Understanding these differences is important to successful microfluidic design. 

Foudeh, A.M., et al., 2012. Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics. Lab Chip 12(18), 3249. Available at http //xhnk.rsc.org/ DOI=c21c40630f. 

Implementation of membrane-less cells, however, faces many inherent problems. Ancillary structures are needed to achieve the laminar flows, including pumps and special channel entrances. The operation is suitable to microfluidic designs with narrow channels and orientation independence... 

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