Microdialysis sampling isoproterenol

M.E. Hadwiger, S.R. Torchia, S. Park, M.E. Biggin and C.E. Lunte, Optimization of the separation and detection of the enantiomers of isoproterenol in microdialysis samples by cyclodextrin-modified capillary electrophoresis using electrochemical detection, J. Chromatogr. B, 1996, 681, 241-249. 

CEEC with methyl-O-P-cyclodextrin as the modifier was used to monitor the pharmacokinetics of (-) and (+) isoproterenol by I.V. microdialysis sampling. Using a combination of peak stacking and electrochemical detection, limits of detection of 0.6 ng/mL were achieved. Isoproterenol has a very short half-life. A flow rate of 1 pL/min was used for the microdialysis sampling. It was possible to analyze the 1 pL samples and monitor the pharmacokinetics of the isoproterenol enantiomers with a temporal resolution of one minute (Figure 14). 

Hadwiger, M.E. Park, S. Torchia, S.R. Lunte, C.E. Simultaneous Determination of the Elimination Profiles of the individual Enantiomers of Racemic Isoproterenol Using Capillary Electrophoresis and Microdialysis Sampling. J. Pharm. Biomed. Anal. 1997 15, 621-629. 

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