Micrococcus pyogenes

Forney has produced a sensitization of guinea-pigs to Micrococcus pyogenes by injection of killed bacillary bodies of this species together with crude wax of tubercle bacillus or M. smegmatis. 

Fry, B. A. Basic triphenylmethane dyes and the inhihition of glutamine synthesis by Staphylococcus aureus (Micrococcus pyogenes var. aureus). J. Gen. Microbiol. 1957,16, 341-349. 

Bioautography vs. Bacterium megatherium or Micrococcus pyogenes var. aureus... 

Bioaulography vs. Micrococcus pyogenes var. aureus or Bacillus subtilis Rf... 

Culture and Inoculum. Use Kl meUa pnewnoniae (P. C. I. 602) or Micrococcus pyogenes var. aureus (A. T. C. C. 6538P) since each of these strains has been observed to respond to less than 1.0 pg. of streptomycin under the conditions of the test. Prepare an actively growing bulk suspension in brain heart infusion and dilute 1 100 in the same medium as described in Section IV, 1. 

Staphylococcal nuclease was initially isolated as an extracellular enzyme (nuclease A) of S. aureus (or Micrococcus pyogenes) (1,49). The extremely high affinity of the nuclease to phosphocellulose at pH 5.8 has provided the basis of convenient purification methods. SNase can also be efficiently purified by the use, as an affinity matrix, of 3 -(4-aminophenylphosphoryl)deoxythymidine 5 -P linked to the CNBr-activated Sepharose (50). 

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