Microbore PTH separations

Protein Sequence Analysis Using Microbore PTH Separations... 

All microbore sequencing runs started Avith two PTH standard nms as described in Methods. Separations were similar for both standards, but the... 

Phenylisothiocyanate (PITC). The use of PITC (Edman Reagent) to form thiohydantoin (PTH) derivatives of amino acids for protein sequencing is well known. The development of reversed phase systems for the separation of these PTH derivatives has resulted in the introduction of both isocratic and gradient elution systems (Fig. 11.2.11). A microbore HPLC unit for PTH-amino acid analysis is now available along with the recently introduced gas phase sequenator... 

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