Microbial adenosine 5’-triphosphate measuring

Holm-Hansen, O., 1973b. Determination of total microbial biomass by measurement of adenosine triphosphate. In L.H. Stevenson and R.R. Colwell (Editors), Estuarine Microbial Ecology. University of South Carolina Press, Columbia, S.C., pp. 73—89. 

PAUL E.A. and JOHNSON R.L. 1977. Microscopic counting and adenosine 5 -triphosphate measurement in determining microbial growth in soils. Applied and Environmental Microbiology, 3, 263-269. 

Metabolites. An overall assessment of microbial activity can be obtained by measuring the amount of adenosine triphosphate (ATP) in field samples. This key metabolite drives many cellular reactions. 

Bjorkman K. and Karl D. M. (2001) A novel method for the measurement of dissolved adenosine and juanosine triphosphate in aquatic habitats apphcations to marine microbial ecology. J. Microbiol. Meth. 47, 159-167. 

Adenosine 5 -triphosphate (ATP) assay The functional significance of ATP in the metabolism of living cells suggests that its measurement should be an excellent monitor of microbial activity in the sample. There are two factors that adversely affect the estimation of bacterial numbers by measurement of ATP first, nonbacterial ATP and, second, quenching of the emitted light. Eailure to destroy the... 

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