Microalgae methanol

Fig. 2.31. Chromatogram of crude lutein from the microalga Chlorella vulgaris by HPLC analysis, A = lutein. Conditions column reversed-phase C18 column (250 X 4.6 mm i.d., 5 pm) mobile phase methanol-dichloromethane-acetonitrile-water (67.5 22.5 9.5 0.5, v/v) flow rate l.Oml/min detection at 450 nm (a). Chromatogram of crude lutein from the microalga Chlorella vulgaris by preparative HSCCC separation, A = lutein. Conditions column multilayer coil of 1.6mm i.d. PTFE tube with a total capacity of 230ml rotary speed 800rpm solvent system ra-hexane-ethanol-water (4 3 1, v/v) mobile phase lower phase (ethanol-water) flow rate lml/min detection at 254 nm sample size 200 mg retention of the stationary phase 58 per cent (b). Reprinted with permission from H.-B. Li el al. [70].

As an assay for antioxidant activity, we examined the inhibition of lipoxygenase activity and radical scavenging activity using the a, a-diphenyl-P-picrylhydrazyl (DPPH) decolorization test. Aqueous, ethanolic and methanolic extracts of microalgae were used in the assays. 

FIGURE6.2 (See color insert.) Mixture of microalgae lipids and methanol in 10 ml view cells... 

Taher et al. (2011,2014b) studied the effect of reaction temperature (35 C-60 C), methanol-to-lipids molar ratios (3 1-15 1), and enzyme loading (10%-50%) on biodiesel production yields from animal fats and microalgae lipids. Process conditions were optimized via response surface methodology. Transesterilication yield of only 40% was obtained when animal fats were used, at 50°C, 200 bar, a 4 1 molar ratio, and a 30% loading of the enzyme (Taher et al., 2011), whereas a conversion above... 

Mg/Al-COs HT Urea method(urea/NO3 molar ratio of 3.0) calcined at 500°C for 4h Refined microalgae oil in methanol... 

---