Microalgae Botryococcus braunii

Gehn F., De Leeuw J. W., Sinninghe Damste J. S., Derenne S., and Metzger P. (1994) The similarity of chemical structures of soluble ahphatic polyaldehyde and insoluble algaenan in the green microalga Botryococcus braunii race A as revealed by analytical pyrolysis. Org. Geochem. 21, 423-435. 

Bayona KCD, Garces LA Effect of different media on exopolysaccharide and biomass production by the green microalga Botryococcus braunii,J Appl Phycol 26(5) 2087—2095, 2014. 

Okada S, DevareimeTP, Chappell J. Molecular characterization of squalene synthase from the green microalga Botryococcus braunii. Race B. Archives Biochem Biophysics 2000 373 307. 

Metzger, P. Largeau, C. Casadevall, E. Lipids and Macromolecular Lipids of the hydrocarbon-rich Microalga Botryococcus Braunii. Progress in the Chemistry of Organic... 

Eroglu, E. and Melis, A. (2010) Extracellular terpenoid hydrocarbon extraction and quantitation from the green microalgae Botryococcus braunii var. Showa. Bioresour. Technol, 101 (7), 2359-2366. 

Lipids and Macromolecular Lipids of the Hydrocarbon-rich Microalga Botryococcus braunii. Chemical Structure and Biosynthesis. Geochemical and Biotechnological Importance... 

Chan Yong, T.P., C. Largeau, and E. Casadevall Biosynthesis of non-isoprenoid hydrocarbons by the microalga Botryococcus braunii. Evidence for an elongation-decarboxylation mechanism. Activation of decarboxylation. Nouv. J. de Chimie 10, 701 (1986). 

Metzger P. and Largeau C. (1999) Chemicals of Botryococcus braunii. In Chemicals from Microalgae (ed. Z. Cohen). Taylor and Francis, pp. 205 - 260. 

The synthesis of microbial fat by bacteria is often ignored because the average fat concentration in dry biomass does not exceed 10%. However, there are strains of Arthrobacter sp., Mycobacterium, and Corynebacterium that are able to accumulate from 30 to 80% lipids in dry matter. Unfortunately, there are other problems related to low growth rates and yields of bacteria, lipid extraction, and the possible allergeiucity and toxicity of the resulting lipids. Microalgae (e.g., Botryococcus braunii and Chlorella pyrenoidosa) serve as attractive sources of PEFA. Dry biomass fat can amount to as much as 85% (Kay, 1991). Moreover, microalgae are a very... 

Mendes et al. investigated supercritical fluid extraction with CO2 on different microalgae. In the case of Botryococcus braunii, the long-chained hydrocarbon components (alkadienes and trienes) could be extracted by means of SCCO2 (at a constant temperature of 40 °C). Studies showed that the solubility of the components in CO2 is dependent on the pressure, and an optimum in terms of yield and extraction rate is attained at 30 MPa. 

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