Michaelis-Menten kinetics parameters

The values of the Michaelis-Menten kinetic parameters, Vj3 and C,PP characterise the kinetic expression for the micro-environment within the porous structure. Kinetic analyses of the immobilised lipase in the membrane reactor were performed because the kinetic parameters cannot be assumed to be the same values as for die native enzymes. 

Use of a N. globerula R-9 strain was demonstrated for desulfurization of straight run diesel oils. Sulfur reduction from 1807 to 741 mg/dm3 was reported at a desulfurization rate of 5.1 mmol/Kgdcw/h. The desulfurization of model oils containing DBT and 4,6 dimethyl DBT was studied and Michaelis-Menten kinetic parameters were reported. 

Lineweaver-Burk analysis using the substrate saturation curves afforded the corresponding Michaelis-Menten kinetic parameters of the reaction V max=l-79 xIO- Ms , KM=21.5mM, kcat = 8.06x 10 s for 69, and Knax = 9.22x 10... 

In conclusion, the values of the Michaelis-Menten kinetic parameters, rmax and KM, can be estimated, as follows ... 

Table 2.5 Estimated Values of Michaelis-Menten Kinetic Parameters for Example 2.3...

Michaelis-Menten kinetic parameters can also be estimated by running a series of steady-state CSTR runs with various flow rates and plotting Cs versus (Cst)/(CSq- Cs). Another approach is to use the Langmuir plot (Csr vs Cs) after calculating the reaction rate at different flow rates. The reaction rate can be calculated from the relationship r = F (CSq - Cs)/V. However, the initial rate approach described in Section 2.2.4 is a better way to estimate the kinetic parameters than this method because steady-state CSTR runs are much more difficult to make than batch runs. 

To determine the Michaelis-Menten kinetic parameters based on initial-rate reactions in a series of batch runs... 

Determine the Michaelis-Menten kinetic parameters as described in this chapter. 

Evaluate the Michaelis-Menten kinetic parameters by employing (a) the Langmuir plot, (b) the Lineweaver-Burk plot, (c) the Eadie-Hofstee plot, and (d) non-linear regression procedure. 

A carbohydrate (S) decomposes in the presence of an enzyme (E). The Michaelis-Menten kinetic parameters were found to be as follows ... 

A substrate is converted to a product by the catalytic action of an enzyme. Assume that the Michaelis-Menten kinetic parameters for this enzyme reaction are ... 

The Michaelis-Menten kinetic parameters for a soluble enzyme for a certain substrate are found to be... 

Another enzyme that was studied extensively in microreactors to determine kinetic parameters is the model enzyme alkaline phosphatase. Many reports have appeared that differ mainly on the types of enzyme immobilization, such as on glass [413], PDMS [393], beads [414] and in hydrogels [415]. Kerby et al. [414], for example, evaluated the difference between mass-transfer effects and reduced effidendes of the immobilized enzyme in a packed bead glass microreactor. In the absence of mass-transfer resistance, the Michaelis-Menten kinetic parameters were shown to be flow-independent and could be appropriately predicted using low substrate conversion data. 

Graphics-based Hiickel molecular orbital calculator of energies and orbitals of TT electrons. EnzymeKinetics for fitting Michaelis—Menten kinetics parameters. ESP (Experimental Section Processor) for organizing synthetic procedures in publication format. LabSystant for evaluating quantitative lab data. Diatomic Molecular Motion and Mechanics. PC-Mendeleev for studying periodic table. SynTree for creating database of reactions. TAPP (Thermodynamic and Physical Properties) database with physical and thermodynamic data on more than 10,000 compounds. PCs and Macintosh. 

Effects of Competitive Inhibitors on and (Michaelis-Menten Kinetic Parameters)... 

Table 1 Michaelis-Menten kinetic parameters for hydrolysis of p-NP-AiaF, p-NP-AraP, p- and o-NP-XylP, and xylobiose.

Figure 4.9-3 A Lineweaver-Burk plot for determining Michaelis-Menten kinetic parameters for ibuprofen esterification using a lipase in SCCO2 according to Scheme 4.9-1 [22].

Table 4.2 Calculated Michaelis-Menten kinetic parameters for WT KS1 and KS1 (N206A) with SNAC 27...

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