Methyl lysine, targeting

Huyen Y, Zgheib O, Ditullio RA Jr, Gorgoulis VG, Zacharatos P, Petty TJ, Sheston EA, Mellert HS, Stavridi ES, Halazonetis TD (2004) Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks. Nature 432 406-411... 

Verschure PJ, van der Kraan I, de Leeuw W, van der Vlag J, Carpenter AE, Belmont AS, van Driel R (2005) In vivo HPl targeting causes large-scale chromatin condensation and enhanced histone lysine methylation. Mol Cell Biol 25 4552-4564... 

Nakayama J, Rice JC, Strahl BD, Allis CD, Grewal SI (2001) Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly. Science 292 110-113 Naumann K, Fischer A, Hofmann 1, Krauss V, Phalke S, Irmler K, Hause G, Aurich AC, Dom R, Jenuwein T, Reuter G (2005) Pivotal role of AtSUVH2 in heterochr EMBO J 24 1418-1429 Nielsen SJ, Schneider R, Bauer UM, Bannister AJ, Morrison A, O Carroll D, Firestein R, Qeary M, Jenuwein T, Herrera RE, Kouzasrides T (2001) Rb targets histone H3 methylation and HPl to promoters. Nature 201(9) 1367-1373... 

A high proportion of the positiveiy charged basic amino acids lysine and arginine within these flexible tails are frequent targets for extensive posttranslational modifications (Berger, 2002). Such modifications include the acetylation of lysine residues, the methylation of lysine and arginine residues, the ubiquitination of lysine residues, the phosphorylation of serine and threonine residues, the sumoylation of lysine residues, and the poly ADP-ribosylation of glutamic acid residues. 

Again, Eu-labeled secondary antibodies are used, for example in an in vitro assay for the PKMT G9a [58] or for cellular hypomethylation of the target of the lysine methyl-transferase S ET7/9 [59]. An alternative is a radioactive assay, in which the biotinylated synthetic peptide substrate is bound to the cavities of avidin-coated microplates. 

---