Methyl hydrogen succinate, hydrolysis

The methanol obtained from the acid hydrolysis of methyl hydrogen succinate in water containing O18 contained none of the heavy isotope 7... 

The ring structure of the ribose residue was ascertained" in the same general manner as for adenosine and guanosine. Triacetyl-dihydrouridine was prepared by the hydrogenation of triacetyl-uridine. On simultaneous deacetylation and methylation this was transformed to the fully methylated dihydro-uridine. By simultaneous hydrolysis and oxidation of this product, with hydrobromic acid and bromine, trimethyl -D-ribonolactone was formed, its identity being confirmed by oxidation to meso-dimethoxy-succinic acid. It follows that the ribose component has the furanose ring structure, and that uridine is 3 -D-ribofuranosyl-uracil. 

Succinic anhydride, 41, 158, 283 o ,/3-bis(triphenyl methyl), MA in synthesis, 198 p-carboxymethylbenzyl, MA in synthesis, 202 commercial preparation, 41, 42 conversion to A2-car boxy anhydride, 225 1,3-dihydronaphthlene substituted, 198 hydrolysis rate, 73, 74 indane substituted, 198 MA hydrogenation product, 236 phosphine reaction, 229 preparation, 15... 

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