Methyl ester, preparation

MetlylEsters. The addition product of two moles of TYZOR TPT and one mole of ethylene glycol, GLY—TI, can be used as a transesterification catalyst for the preparation of methyl esters. The low solubility of tetramethyl titanate has prevented the use of them as a catalyst for methyl ester preparation (488). 

Figure 9.3 Gas chromatogram of methyl esters prepared from milk fat on a 2.4-m stainless steel column, 25% EGS on 42/60 mesh Chromosorb, thermal conductivity detector, injector 325°C, detector 225°C, column 200°C(10).

Garces, R. and Mancha, M. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal. Chem. 211 139-143. 

Figure 4.1 GC/MS analysis of methyl esters prepared from a whole cell lipid extract of the YEpOLEX-PDesat-TnD11Z-transformed ole1 strain of Saccharomyces cerevisiae (A) total ion spectrum of fatty acid methyl esters resolved by capillary GLC (B) mass spectrum of the degradation products of the DMDS adduct of Z11 -16 Me in A. The diagnostic m/z values of the DMDS adduct of Z11-16 Me are labeled. (Reproduced with permission from Knipple et al., 1998. 1998 by The National Academy of Sciences.)...

Figure 4.4 GC/MS analysis of DMDS adducts of methyl esters prepared from whole cell lipid extracts of the YEpOLEX-PoctoZIO-transformed olel strain of S. cerevisiae (A) control olel yeast cells supplemented with 0.5 mM Z11 -18 Acid (B) YEpOLEX-PoctoZl0-transformed olel yeast cells supplemented with 0.05 mM Z11-18 Acid (C) mass spectrum of DMDS adduct of Z10-16 Me in B. GC peaks prior to 25 minutes in A and B correspond to saturated C10-C18 methyl esters. Peaks corresponding to DMDS adducts are found at 44 min for externally added Z11-18 Me and at 38 min in B for Z10-16 Me. (Reproduced with permission from Hao et al., 2002. 2002 by Insect Biochemistry and Molecular Biology.)...

Transfer the methyl esters prepared above into a separatory funnel. Extract twice with 5 mL of petroleum ether. Combine the extracts. 

The methyl esters prepared by oxidation of 96 and 97, respectively, with iodine in methanolic potassium hydroxide were converted into atropine-like drugs by transesterification with 2-(dimethylamino)-... 

Methyl ester prepared from silvex (99% + ), the Dow Chemical Co., 1 microgram per ml. (/igram/ml.) in petroleum ether protect from evaporation losses. 

---