Methyl chloride transferase

Wuosmaa, A.M. and Hager, L.P. (1990) Methyl chloride transferase a carbocation route for biosynthesis of... 

Ni X, Hager LP (1998) cDNA Cloning of Batis maritima Methyl Chloride Transferase and Purification of the Enzyme. Proc Natl Acad Sci U S A 95 12866... 

Wuosmaa AM, Hager LP. Methyl chloride transferase a carboca-tion route for biosynthesis of halometabolites. Science 1990 249 160-162. 

Ni XH, Hager LP. Expression of Batis maritima methyl chloride transferase in Escherichia coli. Proc. Nat. Acad. Sci. USA 1999 96 3611 3615. 

Peter, H., Deutschmann, S., Muellc, A., Gansewendt, B., Bolt, M. Hallier, E. (1989a) Different affinity of erythrocyte glutathione-.S -transferase to methyl chloride in humans. Arch. Toxicol., Suppl. 13, 128-132... 

Lof, A., A. Johanson, M. Rannug, and M. Warholm. 2000. Glutathione transferase T1 phenotype affects the toxicokinetics of inhaled methyl chloride in human volunteers. Pharmacogenetics 10(7) 645-653. 

Warholm, M., A.K. Alexandrie, J. Hogberg, K. Sigvardsson, A. Rannug. 1994. Polymorphic distribution or glutathione transferase activity with methyl chloride in human blood. Pharmacogenetics 4(6) 307-311. 

Thier R, Wiebel FA, Hinkel A, Burger A, Bruning T, Morgenroth K, SengeT, Wilhelm M, Schulz TG. Species differences in the glutathione transferase GSTT1-1 activity towards the model substrates methyl chloride and dichloromethane in liver and kidney. Arch Toxicol 1998 72 622-629. 

Methyl transferases are responsible for methylation of a nucleophile, typically using SAM as the carbon donor. They are known to accept a wide range of nucleophiles such as halides (eq. 1 in Figure 13.22) [64], amines (eq. 2 in Figure 13.22) [65], hydroxyls, and enolates. As expected, the reactivity of methyl transfer to halides follows the order of iodide, bromide, and chloride, with chloride being the poorest acceptor. Methylation of amines in nucleotides and proteins plays important roles in biological activities. 

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