Methods for PEGylation

At present, the most frequently used methods for PEGylation are chemical conjugation between reactive groups in the drug, such as the primary amine of lysine in protein, and end-reactive PEG derivatives, such as the A-hydroxysuccinimide-... 

Other methods for PEGylation include the conjugation of non-natural amino acids, such as p-acetylphenylalanine with mPEG-oxyamine [263,264], pyrrolysinine and pyrroline-carboxy-lysine with mPEG carbonyl compoimds [265], among several other methods [266-268]. 

X. Suo, X. Lu, T. Hu, G. Ma, Z. Su, A sohd-phase adsorption method for PEGylation of human serum albumin and staphylokinase preparation, purification and biochemical characterization. BiotechnoL Lett., 31 (8) 1191-1196,2009. 

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