Method for selecting nutrient medium

PRELIMINARY PROCEDURE METHOD FOR SELECTING NUTRIENT MEDIUM 

The next step is choosing a nutrient medium. Amino acids supplied by high serum concentrations may enhance cell growth significantly in deficient media. As the amount of serum is decreased, the need for a balanced nutrient medium becomes more critical. 

Use the serum lot, serum concentration and cell density chosen in the previous experiment. This should provide the most stringent test of the nutrient balance of the medium. 

Use the type of medium the cells have been growing in as control. Test 3-4 commercially available balanced media, such as F12/DMEM (Ham s nutrient mixture/Dulbecco s modified Eagle s medium), DMEM, IMDM (Iscove s modified Dulbecco s medium), HB-CHO (Hana Biological Chinese hamster ovary medium) or RPMI-1640 (Roswell Park Memorial Institute). 

Use STI if trypsin is used to passage the cells and centrifuge the cells once to remove residual trypsin and STI. 

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