Methanobacterium bryantii

The uptake of nickel by Clostridium pasteurianum and Methanobacterium bryantii has been considered in Section 62.1.7. 

Kirby T. W., Lancaster J. R., Jr., and Fridovich 1. (1981) Isolation and characterization of the iron-containing superoxide dismutase of Methanobacterium bryantii. Arch. Biochem. Biophys. 210, 140-148. 

The source materials for methanogens to form methane are limited to one carbon (Q) compound except for acetate. Previously, Methanobacterium omelianskii was thought to form methane from ethanol (Barker, 1956). But the bacterium was a mixed culture of Methanobacterium bryantii and a bacterium which decomposed ethanol to form hydrogen gas (Bacterium S) (Bryant et al., 1967). Therefore, the... 

Gradient reversed phase HPLC has also been used in the analysis of cobamides present in Methanobacterium bryantii (Whitman and Wolfe, 1984). After initial purification by isocratic reversed phase HPLC at neutral pH the extracts were subjected to gradient HPLC using a C g stationary phase with an initial mobile phase of 100 mM lithium chloride-methanol (76 24) and a final mobile phase of 100 mM lithium chloride-methanol (52 48). Detection by UV absorbance at 254 nm allowed quantitation of as little as 1 pmol of cyanocobalamin. 

Several species have been isolated, studied in pure culture, and taxonomi-cally identified and classified. Some of the notable species are Methanobacterium formicicum, M. bryantii, M. thermoautotrophicum Methanobrevibacter ruminant-ium, M. arboriphilus, M. smithii Methanococcus vannielii, M. voltae Methanomicrobium mobile Methanogenium cariaci, M. marisnigri Methanospirillum hunga-... 

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