Methane monooxygenase hydrocarbon hydroxylation

Liu, K. E., Johnson, C. C., Newcomb, M., and Lippard, S. J., 1993, Radical clock substrate probes and kinetic isotope effect studies of the hydroxylation of hydrocarbons by methane monooxygenase, J. Am. Chem. Soc. 115 939n947. 

A bacterial methane monooxygenase that catalyzes the hydroxylation of saturated hydrocarbons has been isolated, but there are severe limitations in practical use. Similarly the enzymes involved in steroid hydroxylation have been isolated and used in but this practice is not sufficiently well... 

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