Metal interaction chromatography organic solvents

Size exclusion chromatography (SEC, also known as gel permeation chromatography) is a method of separating compounds of different molecular masses and sizes. Because steric interactions between analytes and the stationary phase are relatively weak, unstable forms of metals can be separated from more stable complexes and from adducts stabilized by ionic interactions. Unfortunately, the process of sorption and ionic interactions between the investigated substances and the stationary phase can decrease metal recovery by as much as 50 % these interactions are also responsible for the instability of retention times [146]. The separation can be performed both in the aqueous environment and in the presence of organic solvents. Because the technique is not selective, it is utilized primarily as the first stage of multidimensional chromatography [147]. 

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