Membranes, barrier charge density

Many of the known barriers to transfection may be addressed by custom synthesized lipids. For example, easily biodegradable cationic lipids should (1) enhance TE of CL-DNA complexes in the high membrane charge density regime, where dissociation and release of DNA from the cationic lipid membrane of the complex in the cytoplasm appears to be a barrier to TE, and (2) reduce toxicity in gene silencing applications with CL-siRNA complexes. 

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