Meiotic chromosome preparation

Heyting, C., and Dietrich, A. J. J. (1991). Meiotic chromosome preparation and protein labeling. Methods Cell Biol. 35, 177-202. 

Diploid Cells Culture and Meiotic Chromosome Preparation... 

Figure 26-13 Synaptonemal complexes. (A) Aligned pairs of homologous chromatids lying 0.4 pm apart in Allium cepa. Arrows indicate "recombination nodules" which may be involved in initiating formation of crossovers. Portions of meiotic chromosomes of lily are shown at successive stages (B) Pachytene. (C) Portion of diplotene nucleus. (D) A bivalent at diplo-tene. (E) Two bivalents at diakinesis. Pairs of sister chromatids are coiled with appropriate handedness. (F) Sister chromatid cores are far apart in preparation for separation. A chiasma is present between the two central strands. (B) through (F) courtesy of Stephen Stack.279,279d (G) Pair of sister chromatids coiled with opposite handedness at metaphase. These are immun-ostained with anti-topoisomerase II antibodies. From Boy de la Tour and Laemmli.280 Courtesy of U. K. Laemmli.

Dresser, M. E., and Giroux, C. N. (1988). Meiotic chromosome behavior in spread preparations of yeast. J. Cell Biol 106, 567-573. 

Procedures for Culturing Diploid Cells and Preparation of Meiotic Chromosomes from Dwarf Species of Hamsters ... 

R. Meredith, A simple method for preparing meiotic chromosomes from mammalian testis. Chromosoma 26, 254-258 (1969). 

A. P. Dyban, An improved air-drying method for meiotic and mitotic chromosome preparation from mammalian testes, Tsitologiva 12, 687-690 (1970). 

Protocol 5.2 Methanol-Acetone Eixation Technique I, 92 Protocol 5.3 Methanol-Acetone Eixation Technique II, 93 Protocol 5.4 Eixation with Paraformaldehyde, 94 Protocol 5.5 Eormaldehyde Eixation, 95 Protocol 5.6 Immunostaininc of Eixed Testes Preparations, 96 Protocol 5.7 E-Actin Staining, 97 Protocol 5.8 Chromatin Staining, 98 PREPARATION OE MEIOTIC CHROMOSOMES, 99 Protocol 5.9 Preparation of Meiotic Chromosomes from Adult Testes, 99 Protocol 5.10 Aceto-Orcein Preparation of Meiotic Chromosomes from Earvae OR Pupae, 100... 

Protocol 5.11 Preparation of Unstained Meiotic Chromosomes from Earvae OR Pupae, 101... 

Several techniques have been developed for preparation of male meiotic chromosomes (see, e.g.. Cooper 1965 Ashbumer 1989 Miyazaki and Orr-Weaver 1992). All of these techniques allow a clear visualization of chromosomes, but not of other cellular structures such as the cytoskeleton and the spindle. We describe here three of these techniques that in our hands give good reproducible results. The first technique, developed by Lifschytz and Hareven (1977) and described below in Protocol 5.9, is particularly usefid for the analysis of adult testes. The other two techniques (Ripoll et al. 1985 Cenci et al. 1997) are routinely used in our laboratory for larval and pupal testis preparations. The technique described in Protocol 5.10 is a very simple method for preparing aceto-orcein-stained chromosomes. The other technique outlined in Protocol 5.11 allows preparation of unstained chromosomes that can be subsequently stained with various dyes such as Giemsa, Hoechst 33258, and DAPl, or processed for in situ hybridization. 

Preparation of Meiotic Chromosomes from Adult Testes ... 

Preparation of meiotic chromosomes from testes Protocol 13... 

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