Medium-throughput screening

Lautraite S, Bigot-Lasserre D, Bars R et al. (2003) Optimization of cell-based assays for medium throughput screening of oxidative stress. Toxicol In Vitro. 17 207-220. 

High- and Medium-Throughput Screening Systems for Assaying the Enantioselectivity of Enzymatic Reactions... 

In the case of iterative medium-throughput screening, at any given point in the process, the set of molecules that have been screened thus far is the previously selected set for the next round of screening. In choosing molecules for the next iteration, one may have a selection criterion such as predictive model scores but a diversity criterion may also be applied it is not desirable to screen something identical, or nearly identical, to that which was screened in previous rounds. 

Medium-throughput screening (MTS) Similar to HTS but with only modest throughput requirements which implies more careful usage of robotics and higher quality of data. Typical MTS throughput is 1000 to 10000 compounds. 

NMR (nuclear magnetic resonance) is a versatile technology used in low or medium throughput screening. Different NMR readouts offer a variety of detection modes. The so-called ligand observation mode is used to monitor... 

As mentioned above, subsequent to our initial virtual screen, Vemalis entered a collaboration with a group at the ICR in London. Interestingly, two of the virtual screening hits are closely related to a compound identified by a medium throughput screening (MTS) conducted by the ICR of some 60,000 compounds [30, 31] and show very similar activity. 

Global coverage of metabolome Quantitative Compound identification Large amount of data Q>roblemto lum edata, computer limitalions) Semi-quantitative Medium-throughput screening... 

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