Medium chain fatty acids, engineering

Davies, H.M., Worrell, A.C., Radke, S.E., Hawkins, D.J., DiMento, J. and Voelker, T.A. (1993) Engineering medium chain fatty acid production in oilseeds, in Seed Oils for the Future, eds. S.L. MacKenzie and D.D. Taylor, Americal Oil Chemists Society, Champaign, IL, pp. 155-163. 

Engineering Production of Medium-Chain Fatty Acids... 

Fig. 11. Genetic engineering of rapeseed oil. A high level of lauric acid was achieved by expressing a medium-chain acyl-ACP thioesterase (MCTE) from California Bay in the transgenic seeds. This enzyme intercepts the fatty acid synthesis pathway at 12 carbons and hydrolyzes the fatty acid from its ACP carrier. MoI% of major fatty acids in a typical canola cultivar are compared to the composition achieved through genetic engineering.

Park S J, Park JP, Lee SY, Doi Y (2003) Enrichment of specific monomer in medium-chain-length poly(3-hydroxyalkanoates) by amplification of fadD and fadE genes in recombinant Escherichia coli. Enzyme Microb Technol 33 62-70 Park SJ, Choi JI, Lee SY (2004) Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates. Enzyme Microb Technol 36 579-588 Park JH, Lee KH, Kim TY, Lee SY (2007) Metabolic engineering of Escherichia coli for the production of L-vafine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A 104 7797-7802... 

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