Mechanism cellulase catalysis

Figure 9.14 Proposed reaction mechanisms of cellulase catalysis (A) hydrolysis of cellulose and (B) polymerization of 3-CF into synthetic cellulose. (Reproduced with permission from [250]. Copyright (2005) John Wiley Sons, Inc.).

The monomer design of using j0-CF of two glucose unit structures was important. When j0-glucosyl fluoride of a monosaccharide structure was used, its polymerization by cellulase catalysis produced only cello-oligomers. This indicates that the monosaccharide fluoride is not appropriate enough as a starting monomer compared with j0-CF. The effectiveness of the disaccharide monomer structure j0-CF was considered as a possibile explanation of the cellulose biosynthesis mechanism [33-35]. 

Enzyme s effect mechanism, i.e. enzyme catalysis, operates first of all to form an enzyme substrate complex [14]. Direct physical contact of enzyme and substrate is required to obtain the complex. The current proposed mechanism of cellulase action is illustrated in Fig. 15-1. However, the mechanism of enzymatic hy-... 

Gong C-S, Ladisch MR, Tsao GT (1977) Cellobiase from Trichoderma viride-. purification, properties,kinetics, and mechanism. Biotechnology and Bioengineering 19 959-981 Gong C-S, Ladisch MR, Tsao GT (1979) Biosynthesis, purification and mode of action of cellulase of Trichoderma reesei-. hydrolysis of cellulose mechanisms of enzymatic and acid catalysis. Advances in Chemistry Series vol. 181, American Chemical Society, Washington, DC, pp 261-287... 

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