Measurements peak tailing

There is no practical advantage of one method for measuring peak tailing over another. 

The area of a peak is the integration of the peak height (concentration) with respect to time (volume flow of mobile phase) and thus is proportional to the total mass of solute eluted. Measurement of peak area accommodates peak asymmetry and even peak tailing without compromising the simple relationship between peak area and mass. Consequently, peak area measurements give more accurate results under conditions where the chromatography is not perfect and the peak profiles not truly Gaussian or Poisson. 

The influence of various structural and physicochemical parameters of the stationary and mobile phases on the tailing of a cationic dye in reversed-phase chromatography has been studied in detail. Measurements were performed in a C8 reversed-phase column (80 X 4.6 mm). The isocratic mobile phase was ACN-0.01 M aqueous HC1 (90 10, v/v). Analyses were carried out at 20°C and the flow rate was 1-5 ml/min. The concentration of the cationic dye, l,l -didodecyl-3,3,3, 3 -tetramethylindocarbocyanine perchlorate (Dil) in the model solutions varied between 0.9-309 pM. The dependence of the chromatographic profile of the dye on the injected concentration is illustrated in Fig. 3.112. Calculations and mathematical modelling indicated that the peak tailing of the dye can be... 

Because of some peak tailing, the number of theoretical plates was based on peak width at one-half peak height Ns5.S4 he pooled standard deviation (all temperatures) of retention time measurements (dfs34) was t 0.007 minutes. 

Peak tail currents from the last 30 s of the baseline period averaged and compared to 30 s of data recorded in the presence of each test article solution Current inhibition calculated as percent according to Inhibition (%) = 100 x (7test/ -/baseline), where /test is the peak tail current measured in the presence of the test solution and /baSeiine is the peak tail current measured prior to exposure to the test solution... 

A schematic illustrating the abundance sensitivity is shown in Figure 3.16. Measurements of the ion intensity (7g) of the analyte ion of interest B+ at m - -1 are too high due to peak tailing ((3) of the high intensity (or more abundant) neighbouring peak A+ at mass m. The abundance sensitivity,... 

The abundance sensitivity can also be considered for ions beams with a mass difference of two mass units. For example, the ultrasensitive measurement of is limited by peak tailing from the major uranium isotope and °Sr detection at the ultratrace level is difficult due to peak... 

Foley and Dorsey (1983) have considered the effect of peak tailing on column efficiency and concluded that measuring peak widths at half height (equation (2.26)) substantially overestimates the number of theoretical plates of a tailed peak. Thus resolution (equations (2.46) and (2.47))... 

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