Maxam-Gilbert sequencing technique

Small DNA segments can be synthesized in the laboratory, and commercial instruments are available for automating the work. Sequencing of DNA can be carried out either by the Maxam-Gilbert method, which uses chemical techniques, or by the Sanger dideoxy method, which uses enzymatic techniques. Small amounts of DNA can be amplified by factors of 10 using the polymerase chain reaction (PCR). 

Another sequencing technique is the Maxam-Gilbert method (developed by Alan Maxam and Walter Gilbert). In this method, a single DNA strand is labeled at the 5 end with radioactive phosphorus (P ). The radioactive DNA is divided into four portions and each one is exposed to different chemical reactions. Each reaction causes a 5 cleavage adjacent to either... 

Green et al. Niecke and Flick Scherer and Kuhn Van Zee and Khan Maxam and Gilbert First diphosphene complex made First stable azaphosphenes isolated Correct explanation of the luminescence of white P Introduced rapid DNA sequencing techniques... 

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