Matrigel invasion assay

Cell migration and invasion are cardinal properties of NSCs and CSCs, respectively. Assessing the effect of drugs on these functional parameters is therefore of paramount preclinical relevance. A Matrigel invasion assay, performed in Transwell chambers, represents the most advantageous and efficient method. 

We and others have based invasion assays on preformed tumor spheroids generated either in hanging drops (88) or other nonadherent systems (61). These are then transferred to matrix monolayers or embedded in Matrigel and can be analyzed qualitatively or quantitatively, usually by microscopy and image analysis (see also Chapter 16). A96- or 1,536-well spheroid-based... 

Several evidences demonstrated that evodiamine possesses in vitro and in vivo anticancer properties, such as antiproliferative, anti-metastatic, apoptosis-inducing, and antiangiogenic activities [82]. Evodiamine was able directly to reduce HUVEC capillary tube formation and invasion, using Matrigel assay. In vivo assay, using... 

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