Mathematical derivation of Keq

Derive the mathematical equation that allows you to calculate the equilibrium constant Keq at each guanidine concentration and plot FQ,q = / ([guanidine]. Finally, determine AG° at each guanidine concentration and plot AG0/ = / ([guanidine] and AG0 = f ([Keq]). Calculate from the last graph the value of Keq. 

Do you obtain the same information from both experiments, at 25 and 37°C  

The absorption values of native and denatured /3-galactosidase in the presence of a certain concentration of guanidine are denoted as An and A j, respectively. In the presence of guanidine concentrations where equilibrium exists between the two protein states, the recorded absorbance A(0t,s) is 

A(n) and A(d) denote enzyme absorptions when it is completely native or denatured. 

How do we interpret the results obtained for the equilibrium constant variation Let us consider the following chemical reaction  

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