Manual fractionation protocols

Batchwise kinetic leaching is aimed at understanding both the rate and extent of TE remobilization by changes in environmental scenarios. Moreover, this tecluiique has the potential to overcome the recognized limitation of phase overlapping of several extractants (e.g., the single and sequential extractants of SM T procedures) whenever the several pools attacked feature different kinetic behavior. Contributions of readsorption phenomena to the thermodynamic leachate results may also be discerned by evaluation of kinetic data (Bermond et al., 1998). Manual fractionation protocols based on kinetic explorations are capable of... 

The following protocol is an adaptation of the Ciphergen Biosystems, Inc. user manual and of different published research papers. The Ciphergen Biosystems protocol for serum proteins profiling begins with an anion exchange fractionation step that uses step-wise pH elutions. A total of seven fractions (pH... 

This protocol outlines the general procedure for both manual and automated microwave-assisted peptide synthesis. For the manual synthesis the acyl carrier protein, AGP (65-74) sequence was selected as a representative difficult peptide. The automated synthesis features the preparation of p-amyloid (1 2), another peptide that is known to be difficult to synthesize. Both methods provide the peptides in excellent purity with good yields in a fraction of the time it would take to perform these syntheses using conventional, room temperature synthesis conditions. 

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