Mammalian cells expression

Phospholipase D is widely distributed in bacteria, fungi, plants and animals, and is present in almost all mammalian cells [3]. In mammals, it occurs as alternatively spliced products of two genes (PLD1 andPLD2) (Fig. 3). Most mammalian cells express different levels of both isoforms. Both PLD1 and PLD2 have four conserved sequences (I-IV), and sequences I and IV contain the HXKX4D (HKD) motif that is characteristic of the PLD superfamily, which includes bacterial endonucleases, phospholipid synthases, viral envelope... 

The fl-galactosidase complementation assay has also been adapted for use in mammalian cells (Rossi et al., 1997). The availability of fluorescent substrates for (3-galactosidase allows for fluorescence microscopy and FACS analysis of mammalian cells expressing the fusion proteins of interest. Therefore, similar to the mDHFR system, fl-galactosidase complementation assays may prove useful for genome-scale studies of protein-protein interactions in mammalian cells. 

Ghosh, S., Parvez, M.K., Banerjee, K. et al. (2002) Baculovirus as mammalian cell expression vector for gene therapy an emerging strategy. Molecular Therapy The Journal of the American Society of Gene Therapy, 6(1), 5-11. 

Heterologous expression systems hERG current (whole cell patch clamp) Mammalian cell expression (CHO, HEK-293, mouse L-ceUs, COS-7) and Xenopus oocytes expression system Witchel et al. 77 Zou et al. 78 Cavero et al. 79 Martin et al. 80 McDonald et al.81... 

KvLQTl-minK current (whole cell patch-clamp) Mammalian cell expression (CHO, HEK293) Sanguinetti et al.82... 

Etanercept is produced by recombinant technology in a Chinese hamster ovary mammalian cell expression system. The WCB is grown in a proprietary media system. The cells are cultured initially in flasks and then inoculated into the bioreactor vessel. The product is purified in a number of chromatographic steps, followed by viral inactivation and viral filtration steps. 

EPOGEN /procrit (epoetin alfa) [mammalian cell-expressed glycoprotein]... 

Erythropoietin, a 34-39 kDA glycoprotein, was the first human hematopoietic growth factor to be isolated. It was originally purified from the urine of patients with severe anemia. Recombinant human erythropoietin (rHuEpo, epoetin alfa) is produced in a mammalian cell expression system... 

CHO cells, using an inducible expression system [100], and in HEK cells [97]. While the recombinant channels show the expected pharmacology, some of the biophysical properties differ from native TTX-resistant currents, suggesting the existence of auxiliary subunits. In Xenopus oocytes and in mammalian cells, expression of rNayl.8 was increased substantially by co-expression with Navbl and Navb3, respectively [98,101]. 

CH2 and CH3) portion of human immunoglobulin G1 (IgGl) produced in a mammalian cell expression system (anti-CTLA-4)... 

An interesting mutation in GPIIIa that results in the expression of a phenotype identical to the phenotypes of the GPIIb mutations described above is a L143W(L117W) mutation . This mutation identified in patient MK (Figures 1 and 3) has been shown by mammalian cell expression studies to result in the intracellular retention of misfolded GPIIb/IIIa heterodimeric complexes. 

Most mammalian cells express more than one type of NT, often combining members of both gene families, CNT and ENT, in a single cell type. Transcripts for CNTl,... 

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