Mammalian cell culture systems microcarriers

Three particle collision mechanisms can occur in an agitated vessel. These are (a) particle-vessel, (b) particle-impeller and (c) particle-particle. Most of the work on collisions has been related to secondary nucleation, but there are other systems where mechanical abrasion following impact may occur and may be undesirable, e.g. breakdown of friable catalysts, or in mammalian cell culture on microcarriers or desirable, e.g. removal of an impervious outer skin which forms on ore particles during some leaching processes. 

Reuveny S (19 ) Microcarrier culture systems. In Lubiniecki AS (ed.) Large-scale Mammalian Cell Culture Technology, pp. 271-341. Marcel Dekker, New York. 

Commercial scale cultivation of mammalian cells is accompHshed using different technologies roller bottles, microcarriers, suspension (batch, fed-batch or perfusion mode) and hollow fiber bioreactors (Table 2). However, especially for products needed in large amounts, suspension cultivation seems to be the most effective system [4, 5]. Suspension-based systems are characterized by a homogeneous concentration of cells, nutrients, metabolites and product, thereby facilitating scale-up [6] and enabling an accurate monitoring and control of the culture [7]. 

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