Malate dehydrogenase beef heart

Many of the biochemical processes that generate chemical energy for the cell take place in the mitochondria. These organelles contain the biochemical equipment necessary for fatty acid oxidation, di- and tricarboxylic acid oxidation, amino acid oxidation, electron transport, and ATP generation. In this experiment, a mitochondrial fraction will be isolated from beef heart muscle. The mitochondria will be analyzed for protein content and fractionated into submitochondrial particles. Each fraction will be analyzed for malate dehydrogenase and monoamine oxidase activities. [Pg.357]

Students will isolate intact mitochondria from beef heart and fractionate them to prepare submitochondrial particles. Each fraction will be characterized by protein estimation by the biuret method and measurement of malate dehydrogenase and monoamine oxidase activity. [Pg.361]

Malate dehydrogenase has been identified in a wide variety of sources and purified to homogeneity from a number of them. The majority of studies have been carried out with enzyme isolated from either pig or beef heart. The first apparently pure preparation was obtained by Wolfe and Neilands (7). It differs from previously reported procedures (S,9)... [Pg.370]

Purification of the malate dehydrogenase isozymes from beef heart have utilized similar procedures (17-22) to those already described for... [Pg.371]

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