Main Features of RPLC Approaches

Based on the partitioning of the solute between a polar mobile and an apolar sta-honary phase (RPLC), the chromatographic data were expressed as retention factors (logfe) given by  

However, some authors also used additional terms (correction term or addihonal variable) and therefore, the general correlation is given by  

In prachce, coefficients a and b (and c) must be determined for every couple of a stationary phase and a mobile phase (and sometime for a given class of compounds) using retenhon factors of reference compounds of known log Poet-Then this calibration equation can be used to determine log Poet of novel compounds. 

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