Magnetic beads sample fractionation

In a 2.0- or 1.5-ml microcentrifuge tube, combine the amplified DNA, or prespun product from the optional step, with 100 pi of well-suspended magnetic bead slurry. Place on a rotator or agitate gently by hand for 15-30 min. Remove the liquid. Save at least a 10-pl sample for later analysis of capture efficiency on an agarose gel. (Typically, some dsDNA is visible in this supernatant fraction. Only a very bright band, combined with low yield of ssDNA, should be of concern.)... 

Fig. 2. Mass spectra (m/z 1000-10,000) acquired by MALDI-TOF/TOF MS in linear mode using serum sample fractionated by IMAC-Cu and hydrophobic C8 magnetic beads. The serum sample was fractionated using IMAC-Cu magnetic beads first and then the unbound solution was transferred to C8 magnetic beads for additional fractionation (tandem chromatography). Different profiles are generated using this tandem chromatography method.

Fig. 1. Magnetic separation of NK cells characterized by CD56 cell surface marker. FACS histograms of the PBL sample labeled with anti CD56-PE primary antibody and anti PE-MACS bead secondary (A), and the negative (B), and positive (C) fractions from the magnetic separation experiment as described in the text. Note enrichment of the CD56+ cells in the positive cell fraction.

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