Magnetic beads elution from

Add 30 pL of TE to each well then remove plate from magnet and spin down at a speed of 82.3g wait 30 min before proceeding to next step to allow DNA to elute from beads. 

Place the tubes in a magnetic beads separator and wait for about 20 s to separate the beads from the elution solution at the wall of the tubes. 

The process of moving the microcentrifiige tube from the 80 °C heat block to the magnetic stand, and subsequent recovery of the eluted RNA into a new tube, should take no more than 10 s. This is to minimize rebinding of the polyadenylated RNA to the beads once the tube has cooled. 

Elute the protein-DNA complexes from the beads by adding 100 pL cold elution buffer, vortex vigorously, incubate for 1 min at 37 °C and pellet the beads with a magnetic stand. Transfer the supernatant (eluted) to 1.5 mL microcentrifuge tube. Add 50 pL of 1 M Tris-HCl, pH 9, to neutralize. Repeat the elution and neutralization twice more, with the last elution step being performed for 4 min at 37 °C. The combined volume of eluate will be 450 pL. 

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