MAGIChips

MAGIChip is a generic microchip-size device, where spots of polyacrylamide gel are separated by hydrophobic glass. Several gel squares (100 pm) are contained on one glass support, and are separated by 200 pm. Short DNA sequences are immobilized on the gel and are assayed for hybridization later, the matrix compound is added, allowed to dehydrate, and the spots are analyzed by MALDI-TOF. With this device, different SNP sequences are recognized by their different masses. 

Gel pads of MAGIChips could serve as micro cuvettes, which would enable massive parallel or multiplex thermodynamical studies for any DNA duplexes, triplexes, DNA binding ligands, proteins, and so forth. Thousands of melting curves could be obtained simultaneously, which cannot be accomplished with the usual UV-VIS spectrophotometer. This fact demonstrates that all mismatch discrimination and mutation detection is based on the sohd theoretical foundation of classical DNA thermodynamics. 

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