Macromolecular substances, affinity

AFFINITY CHROMATOGRAPHY OF MACROMOLECULAR SUBSTANCES ON ADSORBENTS BEARING CARBOHYDRATE LIGANDS... 

Affinity chromatography has become the method of choice for purifying macromolecular substances and for determining the nature of the stereospecific interactions of these substances.1,2 The method has... 

Affinity adsorbents having carbohydrate ligands have been used for the isolation and purification of many types of macromolecular substances. To illustrate, several types of antibodies, enzymes, lectins, and myeloma proteins have been obtained in highly purified form. All of the substances that have been purified by this procedure exhibit specificity for a particular carbohydrate moiety. It is the purpose of this article to assemble information on the methods for preparing affinity adsorbents having carbohydrate ligands, and to illustrate the use of these adsorbents for the purification of representative, macromolecular substances. 

The model used in the above studies ignores the departure from the bulk density of the adsorbate brought about by the interaction of the two interfaces. Li and Caldwell s article addresses this issue by introducing a three-component model consisting of a core particle, a flexible macromolecular substance with affinity toward the particle, and a solvation shell (see Fig. lb). 

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