Macrolides individual drugs

The HPLC-receptorgram assay combined the advantages of HPLC separation with the multiresidue detection of the Charm II tests. The procedure was tested for identification and quantitation of the most common veterinary drugs at regulatory levels or lower. It was validated for 40 individual drugs from seven antibiotic families 10 /3-lactams, 13 sulphonamides, 8 tetracyclines, 4 macrolides, 3 amphenicols, and other miscellaneous antimicrobials. This procedure combined a simple aqueous extraction and SPE with HPLC fractionation of individual drugs. Final identification and quantitation was achieved with the Charm II test. A drug contaminant could be identified in less then 3 hours (50). 

Our interest has focused on individual origin, assemblage of these three resistant genes, and the regulation mechanism of macrolide resistance in the plasmid pMS97 Where has each of them come from Have they ever constituted a cluster themselves How do they regulate the expression of drug resistance, independently or cooperatively ... 

Resistance to the macrolides in gram-positive organisms involves production of a methylase that adds a methyl group to the ribosomal binding site. Resistance in enterobacteria is the result of formation of drug-metabolizing esterases. Cross-resistance between the individual macrolides is complete. 

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